Online determinations of serotonin in awake rat striatum by microdialysis. Shown are the first eight hours of a 16-hour run. Each point represents a chromatogram (typical basal chromatogram shown in the inset).  Zhang et al. 2013

Online determinations of serotonin in awake rat striatum by microdialysis. Shown are the first eight hours of a 16-hour run. Each point represents a chromatogram (typical basal chromatogram shown in the inset). Zhang et al. 2013

Online neurotransmitter monitoring with fast capillary liquid chromatography

Goal: Continuous, fast, liquid chromatographic separations on nL samples with nM or lower quantitation for neurotransmitters.

Approach: cLC system is coupled online to in vivo microdialysis. Speed and sensitivity benefit from high temperature and pressure (Liu et al., 2010).

Results: Quantitative, continuous, online determination of serotonin and dopamine (Gu et al. 2015) in dialysate from rat brain once per minute.